
IDEXX BioAnalytics now provides high quality LC-MS/MS bioanalysis
for small molecule compounds. In addition to standard protein
precipitation, IDEXX BioAnalytics offers solid phase extraction and
solid supported liquid extraction.
We offer the same high-quality, flexible, and fast turnaround in our bioanalysis laboratory as we do for our current clinical and anatomic pathology services.
Analytical Equipment
• Sciex 5500+ Triple Quadrupole Mass Spectrometer
– ESI source and APCI source
• Shimadzu Nexera X2 HPLC
• Integra Biosciences VIAFLO96 pipetting robot
• Beckman Avanti JXN-26 refrigerated centrifuge
• Mettler XPR36DR analytical balance
• Biotage TurboVap96 evaporator
• WinNonlin Phoenix software
Extraction Types
• Protein precipitation
– Organic and acidic methods
• Solid Phase Extraction
• Solid Supported Liquid Extraction
Sample Types We Work With
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Blood
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Serum
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Urine
-
Tissue homogenates
Bioanalytical Services: Small molecule LC-MS/MS Services
Method Feasability
• Detecting the compound in the mass spectrometer
– Compound infusion
– Ionization parameter optimization
• Eluting the retained compound from the UHPLC column
– Column and mobile phase selection
– Compound injection for retention time
and peak shape assessment
Development and Pre-qualification
• Optimizing the LC-MS/MS method
• Accuracy
– 3 concentrations tested
• Precision
– 3 concentrations tested
– Quality control samples every 10 samples
• Linearity
– 8 concentrations tested
• Sensitivity
– Limit of Detection
Method Qualification
Sensitivity and Linearity
• Assessment of duplicate extracted and non-extracted
standard curves for linearity and sensitivity and
reproducibility on two or three different injection days
Quality Controls
• High, medium, and low concentrations
in duplicate
Non-GLP Sample Analysis
• Pre- and post-analysis standard curves for QC
• Interspersed QCs through samples
– 10% of total number of samples tested
• Summary report in Excel format
– System suitability
– Standards & QC: Reporting of used/excluded,
nominal, back calculated, accuracy, coefficient
of variation
– Carryover
– Samples: Calculated concentration in tabular
format, graphical format optional
IDEXX BioAnalytics provides the expertise to best support your Small Molecule Bioanalysis work.
Contact IDEXX BioAnalytics today to discuss your research needs.