Extraction Types

• Protein precipitation
      – Organic and acidic methods
• Solid Phase Extraction
• Solid Supported Liquid Extraction

Sample Types 

• Blood
• Serum
• Urine
• Tissue homogenates

Analytical Equipment

Method Feasibility

Test Code: 63489

Feasibility for Novel Compounds

• Compound infusion, ionization, and parameter optimization
• UHPLC elution for retention time and peak shape assessment
• Extraction method efficiency after protein precipitation or other methods
  

Development and Pre-Qualification

• Accuracy determined at 3 concentrations
• Precision determined at 3 concentrations
• Linearity determined at 8 concentrations
• Limit of Detection identified

Method Optimization

Test Code: 63513

Optimization of Known Compounds

• Utilizes published or client-provided methods
• Compound detection with UHPLC-mass spectrometer
• Extraction method testing for maximum efficiency

Development and Pre-Qualification

• Accuracy determined at 3 concentrations
• Precision determined at 3 concentrations
• Linearity determined at 8 concentrations
• Limit of Detection identified

Method Qualification

Test Code: 63487

Method Reproducibility and Robustness

• Duplicate extracted standard curves and quality control samples on 3 different days
• Determination of linearity, sensitivity, accuracy, and precision
• Optional add-ons: Freeze/thaw, storage stability, specificity

Sample Analysis

Test Code: 63510

Specimen Evaluation

• Pre- and post-analysis standard curves for 8+ concentrations
• Summary Excel report includes statistics, accuracy, CV, and carryover with sample data
• Interspersed quality control samples to be 10% of tested samples
• Externally generated data analysis and interpretation available