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      Pharmacokinetics

      Advanced LC-MS/MS Bioanalysis for Small Molecules

       

       

      DMPK - 700x400

      Cutting-edge LC-MS/MS Bioanalysis for Small Molecule Compounds

      Count on us to deliver with unmatched speed, precision, and adaptability. IDEXX BioAnalytics provides high-quality and flexible LC-MS/MS bioanalysis for small molecule compounds with a fast turnaround. In addition to standard protein precipitation, we offer solid phase extraction and solid-supported liquid extraction. We offer the same high-quality, flexible, and fast turnaround in our bioanalysis laboratory as we do for our current clinical and anatomic pathology services.

      Rely on our swift, accurate, and versatile approach to exceed your needs.

      Extraction Types

      • Protein precipitation
            – Organic and acidic methods
      • Solid Phase Extraction
      • Solid Supported Liquid Extraction

      Sample Types 

      • Blood
      • Serum
      • Urine
      • Tissue homogenates

      Analytical Equipment

      • Sciex 5500+ Triple Quadrupole Mass Spectrometer
      • ESI source and APCI source
      • Shimadzu Nexera X2 HPLC
      • Integra Biosciences VIAFLO96 pipetting robot
      • Beckman Avanti JXN-26 refrigerated centrifuge
      • Mettler XPR36DR analytical balance
      • Biotage TurboVap96 evaporator
      • WinNonlin Phoenix software

      Bioanalytical Small Molecule LC-MS/MS Services

      Method Feasibility

      Test Code: 63489

       

      Feasibility for Novel Compounds

      • Compound infusion, ionization, and parameter optimization
      • UHPLC elution for retention time and peak shape assessment
      • Extraction method efficiency after protein precipitation or other methods

      Development and Pre-Qualification

      • Accuracy determined at 3 concentrations
      • Precision determined at 3 concentrations
      • Linearity determined at 8 concentrations
      • Limit of Detection identified

      Method Optimization

      Test Code: 63513

       

      Optimization of Known Compounds

      • Utilizes published or client-provided methods
      • Compound detection with UHPLC-mass spectrometer
      • Extraction method testing for maximum efficiency

       

      Development and Pre-Qualification

      • Accuracy determined at 3 concentrations
      • Precision determined at 3 concentrations
      • Linearity determined at 8 concentrations
      • Limit of Detection identified

      Method Qualification

      Test Code: 63487

       

      Method Reproducibility and Robustness

      • Duplicate extracted standard curves and quality control samples on 3 different days
      • Determination of linearity, sensitivity, accuracy, and precision
      • Optional add-ons: Freeze/thaw, storage stability, specificity

      Sample Analysis

      Test Code: 63510

       

      Specimen Evaluation

      • Pre- and post-analysis standard curves for 8+ concentrations
      • Summary Excel report includes statistics, accuracy, CV, and carryover with sample data
      • Interspersed quality control samples to be 10% of tested samples
      • Externally generated data analysis and interpretation available

      Small molecule bioanalysis sample collection & shipping

      Comprehensive information on procedures for sample collection, detailed instructions on the packaging and shipping process, and additional details for small molecule bioanalysis.

      Sample Requirements - Icon

      Sample Requirements

      Samples must consist of the same matrix that was tested in the Method Feasibility or Method Optimization study. The accepted sample types include: plasma, serum, urine, and tissue homogenates.

       

      Collection Instructions - Icons

      Collection Instructions

      Generally, the sample size should be 150-500 µL. Please consult your Method Feasibility or Method Optimization study report for more details.

       

      Sample Storage - Icon

      Sample Storage

      Samples should be kept frozen (≤-20°C) to maintain sample integrity. Do not thaw.

      Shipping - Icon

      Shipping

      Ship samples on dry ice to keep frozen and maintain sample integrity.

      Please ship samples to IDEXX BioAnalytics North Grafton, MA lab. 

      Turnaround Time - Icon

      Turnaround Time

      Turnaround time is dependent on scheduling the study. Sample analysis reports are typically sent out within 5 business days of the scheduled analysis date.

      Additional Information - Icon

      Additional Information

      Please provide the analyte and its internal standard (≥10 mg) for the standard and quality control manufacture.

      Biological matrices can be provided for certain species (plasma, serum, urine); others may incur an additional fee.

      A redacted report sample is available upon request.

      Fast, accurate, and reliable results from the team you know and trust.

      Connect with IDEXX BioAnalytics today.