For submission of live fish for necropsy: Please ship live fish in clearly labeled heavy-duty plastic bladders or in doubled plastic bags, secured so that they will not leak and containing approximately 1/3 system water and 2/3 air. Fish should be shipped in insulated containers by overnight courier.
For submission of fish for PCR only: Please submit frozen fish with sufficient ice packs to ensure that fish remain frozen during transit. Dry ice is not required.
For submission of fish embryos or larvae for PCR: Please euthanize embryos and submit frozen on ice or cold packs. Dry ice is not required.
For submission of fish for histopathology only: Please either submit fish fixed in 10% buffered formalin or another formalin-based fixative such as Dietrich’s, Davidson’s, or Bouin’s fixative (please indicate). To ensure proper fixation, open up the coelom (abdomen). This can be accomplished by making a lengthwise incision through the body wall or by removing a small piece of the body wall. Fish cannot be pooled for histopathology. Samples may be shipped at ambient temperatures by overnight courier.
For submission of fish for both histopathology and PCR from an individual fish: Please submit fish that have been frozen flat in liquid nitrogen or at -80°C. Please package and label each fish individually (e.g., by wrapping each fish flat in aluminum foil), and then place the individually packaged and labeled fish into a larger, rigid plastic container such as a large conical tube. Samples should be shipped on dry ice by overnight courier.
Environmental samples and feeds
For submission of detritus (biofilm or sediment) for PCR: Please collect 1 mL of detritus into a tube and submit frozen with cold packs. Dry ice is not required. Collection of detritus is recommended over environmental swabs.
For submission of fish feces for PCR: Please collect a pool of fish feces into a microcentrifuge tube and submit frozen with cold packs. Dry ice is not required.
For submission of live feed cultures for PCR: Please submit at least 1 mL of live feed cultures (e.g.,Artemia, rotifer, orParamecium) frozen with cold packs. Dry ice is not required.
Samples for bacterial or fungal culture
For submission of culture swabs: Please submit culture swabs collected from skin, gills, or lesioned organs in a culturette containing a suitable transport medium, such as Amies Medium without charcoal. Swabs should be shipped with cold packs in an insulated container by overnight courier.
For identification of bacterial and fungal cultures by MALDI-TOF mass spectrometry: Please submit cultures on agar plates wrapped in Parafilm®, or submit cultures on culture swabs with a suitable transport medium, such as Amies Medium without charcoal. Swabs and plates should be shipped with cold packs in an insulated container by overnight courier.
For culture of water samples: Please submit 100 mL of water in a plastic container with a tight-fitting lid that will not leak in transit. Water samples should be shipped with cold packs in an insulated container by overnight courier.