Q In a qPCR assay using probe, would non-specific amplification be possible occur in a situation of very high concentration of non-target DNA? 

During specificity test of a diagnostic, when it challenged against plasmid DNA from a non-target species at a very high concentrations of DNA non-target (10E8 copies/ul) there was amplification. However, at lower concentrations this did not occur. Non-specific amplification may have occurred due to stochastic events, ie, since all the components necessary for amplification to occur occur (except for the target DNA), the physical interaction between the molecules could have favored non-specific amplification, considering this extreme situation of High concentration of non-target DNA? 

Corynebacterium, wow!

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Opti-Spot Instructional Video

The Industry Gold Standard

Dr. Matthew Myles from IDEXX BioResearch invented, validated, and patented Opti-Spot® for health monitoring of animal colonies.  Opti-Spot® is still the simplest, greenest, and most economical method to obtain sensitive and specific serologic data.

INTRODUCTION:

Your “how to” blog post should teach the reader how to do something by breaking it down into a series of steps.

Begin your blog post by explaining what problem you are going to solve through your explanation and be sure to include any relevant keywords. Add in a personal story to establish your credibility on this topic. And make sure to end your blog post with a summary of what your reader will gain by following your lead.

Need some inspiration? Check out these "How-To" examples from the HubSpot blog:

• How to Write a Blog Post: A Simple Formula to Follow
• How to Do Keyword Research: A Beginner's Guide
• How to Write an Effective Meta Description (Yes, They Still Matter)

Q In a qPCR assay using probe, would non-specific amplification be possible occur in a situation of very high concentration of non-target DNA? 

During specificity test of a diagnostic, when it challenged against plasmid DNA from a non-target species at a very high concentrations of DNA non-target (10E8 copies/ul) there was amplification. However, at lower concentrations this did not occur. Non-specific amplification may have occurred due to stochastic events, ie, since all the components necessary for amplification to occur occur (except for the target DNA), the physical interaction between the molecules could have favored non-specific amplification, considering this extreme situation of High concentration of non-target DNA? 

Opti-Spot Instructional Video

The Industry Gold Standard

Dr. Matthew Myles from IDEXX BioResearch invented, validated, and patented Opti-Spot® for health monitoring of animal colonies.  Opti-Spot® is still the simplest, greenest, and most economical method to obtain sensitive and specific serologic data.

Corynebacterium, wow!

Cornye,am l;scmas;klfjzlvjzovzco jzpcjPIOc xjOI c ZOIc jOi jzxoicj zxoicj zxo icjz oxic jzoixvcjzoxivjczoxivjuz pxovjzp oxvj czxoich OIxch OIchOIZchz oc jxoic jzxlc ijoisdyos hoZIhjcjcl j.

The Implications of Rat Polyomavirus 2

RPyV2 can cause disease and mortality in immunodeficient animals. Further population surveys found antibodies to this novel virus in 32% of immunocompetent rats used in biomedical research. We’ve developed and validated both serologic and molecular diagnostic assays for RPyV2 as stand alone tests that can also be added to any profile.